Anatomy of a negative feedback loop: the case of IkB
analysis of wt, IkBa2/2, IkBa2/2þ pBabe_IkBa, and IkBa2/2þ 5xkB_ IkBamurine embryo fibroblast (MEF) cell lines. EMSA indicates NFkB activity over a 120 min time course after stimulation with 1 ng ml21 of TNF; NFY binding was used as an EMSA control. Western blot shows protein abundances for IkBa, IkBband IkBewith a-tubulin as a loading control.
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